Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The polymorphism of HLA class II molecules in man is particularly evident when comparisons between population groups are made. This study describes a DR3 haplotype commonly present in the American black population. Unlike the Northern European population in which almost all DR3 individuals are DQw2, approximately 50% of DR3-positive American blacks express a serologically undefined DQ allelic product. DNA restriction fragment analysis with the use of several unrelated individuals and an informative family has allowed us to identify unique DQ alpha- and beta-fragments associated with the DR3, DQw- haplotype. Based on fragment size, the DQ alpha genes of the DR3, DQw- and DRw8, DQw- haplotypes are similar as are the DQ beta genes of DR3, DQw-; DRw8, DQw-; and DR4, DQw- haplotypes. In addition, a DX beta gene polymorphism has been identified which is associated with some DR3 haplotypes including the American black DR3, DQw- haplotype. cDNA sequence analysis has revealed a DQw2-like alpha gene and a DQ beta gene which is similar to that previously described for a DR4, DQw- haplotype. It is postulated that recombination between DQ alpha and DQ beta genes and between the DQ and DX subregions has generated the various DR3 haplotypes and has played an important role in creating diversity in the HLA-D region.

Type

Journal article

Journal

Journal of immunology (Baltimore, Md. : 1950)

Publication Date

02/1988

Volume

140

Pages

885 - 892

Addresses

Department of Microbiology, Georgetown University, Washington, DC 20007.

Keywords

Humans, DNA Restriction Enzymes, Deoxyribonucleases, Type II Site-Specific, DNA, HLA-D Antigens, HLA-DQ Antigens, HLA-DR Antigens, HLA-DR3 Antigen, Cloning, Molecular, Recombination, Genetic, Amino Acid Sequence, Haplotypes, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, Molecular Sequence Data, United States, Black or African American, Black People