Probing the proteasome cavity in three steps: bio-orthogonal photo-reactive suicide substrates
Geurink PP., Florea BI., Van der Marel GA., Kessler BM., Overkleeft HS.
Tri-functional activity-based proteasome probes are used for a three-step chemical proteomics approach, in which proteasomal catalytic sites are covalently and irreversibly modified, followed by photocrosslinking of these to flanking subunits and Staudinger–Bertozzi ligation for visualization and identification of the resulting conjugates.