Multi-trait genome-wide association study identifies new loci associated with optic disc parameters.
Bonnemaijer PWM., Leeuwen EMV., Iglesias AI., Gharahkhani P., Vitart V., Khawaja AP., Simcoe M., Höhn R., Cree AJ., Igo RP., International Glaucoma Genetics Consortium None., NEIGHBORHOOD consortium None., UK Biobank Eye and Vision Consortium None., Gerhold-Ay A., Nickels S., Wilson JF., Hayward C., Boutin TS., Polašek O., Aung T., Khor CC., Amin N., Lotery AJ., Wiggs JL., Cheng C-Y., Hysi PG., Hammond CJ., Thiadens AAHJ., MacGregor S., Klaver CCW., Duijn CMV.
A new avenue of mining published genome-wide association studies includes the joint analysis of related traits. The power of this approach depends on the genetic correlation of traits, which reflects the number of pleiotropic loci, i.e. genetic loci influencing multiple traits. Here, we applied new meta-analyses of optic nerve head (ONH) related traits implicated in primary open-angle glaucoma (POAG); intraocular pressure and central corneal thickness using Haplotype reference consortium imputations. We performed a multi-trait analysis of ONH parameters cup area, disc area and vertical cup-disc ratio. We uncover new variants; rs11158547 in PPP1R36-PLEKHG3 and rs1028727 near SERPINE3 at genome-wide significance that replicate in independent Asian cohorts imputed to 1000 Genomes. At this point, validation of these variants in POAG cohorts is hampered by the high degree of heterogeneity. Our results show that multi-trait analysis is a valid approach to identify novel pleiotropic variants for ONH.